Register to publish
Log In
by Bhoj Singh
in Noto-are Medicine, October 10, 2017. (19963917)
Metallo-B-lactamase (MBL) producing bacteria are one of the most dangerous emerging threats to public health professionals. In the laboratory, the production of MBL by any bacteria is determined using double disc synergy (DDS) test (one carbapenem disc and other EDTA disc) placed at a distance of 15-20 mm while testing sensitivity for other antimicrobials or using MBL E-Test strips available from Biomerieux and other producers. In our observations, we found DDS more efficient and giving clear results while chances of mistakes exist in observation of epsilon zone of growth inhibition with E-test on carbapenem-resistant EDTA sensitive bacteria. Here photo-plates are shown to make it clear using a carbapenem-resistant Streptococcus equisimilis isolated from a case of urinary tract infection (UTI) in a zoo lioness. As a cause of UTI S. equisimilis has rarely been reported, that too in a lioness.
E-test for determining MIC of imipenem, cefotaxime, colistin and cefotaxime+clavulanic and to detect MBL potential of Streptococcus equisimilis isolate from a lioness case of UTI
Antibiotic sensitivity assay and Double disc synergy test for Streptococcus equisimilis isolate from a lioness case of UTI