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by Bhoj Singh
in Noto-are Medicine, October 10, 2017. (19963917)
Metallo-B-lactamase (MBL) producing bacteria are one of the most dangerous emerging threats to public health professionals. In the laboratory, the production of MBL by any bacteria is determined using double disc synergy (DDS) test (one carbapenem disc and other EDTA disc) placed at a distance of 15-20 mm while testing sensitivity for other antimicrobials or using MBL E-Test strips available from Biomerieux and other producers. In our observations, we found DDS more efficient and giving clear results while chances of mistakes exist in observation of epsilon zone of growth inhibition with E-test on carbapenem-resistant EDTA sensitive bacteria. Here photo-plates are shown to make it clear using a carbapenem-resistant Streptococcus equisimilis isolated from a case of urinary tract infection (UTI) in a zoo lioness. As a cause of UTI S. equisimilis has rarely been reported, that too in a lioness.
by Bhoj Singh
in Noto-are Medicine, July 19, 2014. (17223318)
In the study antimicrobial activity of adonitol, arabinose, cellobiose, dulcitol, galactose, glycerol, glucose, inositol, lactose, maltose, mannitol, mannose, mellibiose, raffinose, salicin, sorbitol, sucrose, trehalose and xylose was determined on 96 strains of Bacillus anthracoides, 3; Bacillus badius, 5; Bacillus brevis (3), Bacillus circulans (4), Bacillus coagulans (3), Bacillus lentus (6); Bacillus marcerans (3), Bacillus pantothenticus (15), Citrobacter amalonaticus (3), Citrobacter diversus (1), Enterobacter agglomerans (2), Enterobater gregoviae (1), Enterococcus asacchrolyticus (1), Enterococcus avium (4), Enterococcus caecorum (8), Enterococcus gallinarum (2), Enterococcus malodoratus (1), Enterococcus mundtii (1), Enterococcus raffinosus (4), Ervinia ananas (1), Klebsiella oxytoca (2), Microcccus luteus (2), Morganella morganii (2), Proteus mirabilis (9), Proteus vulgaris (6), Providencia rettgeri (3) and Pseudomonas aeruginosa (1). Of the 19 sugars tested on 96 strains only glycerol, lactose, maltose, mannitol, raffinose and xylose had bacteriostatic effect against 1, 1, 6, 4, 4 and 3 strains, respectively... more
by Bhoj Singh
in Noto-are Science, March 1, 2017. (12435147)
Though cow is a sacred animal in India, is dying an unnatural death in India. The quest is who is responsible for the unnatural death of cow progeny in India, dairying practices, veterinary services, vaccination with substandard vaccines, veterinary administration involved in vaccine and drug quality monitoring or anyone else. Diseases including foot and mouth disease (FMD), hemorrhagic septicemia (HS), black leg/ black quarter (BQ), anthrax and John's disease (JD) are the common killers prevailing since ages in India despite the availability of vaccines and other measures to control and stamp out these.
by Bhoj Singh
in Noto-are Medicine, July 17, 2017. (17296246)
Objective: The study was conducted with single objective, to understand effect of foot and mouth disease (FMD) on secondary sex ratio (SSR) in dairy animals including cattle (Bos indicus, Tharparkar; Bos indicus + Bos taurus, Vrindavani) and Murra buffalo (Bubalus bubalis) maintained at Indian Veterinary Research Institute, Izatnagar. Design: Retrospective data for six years of IVRI, Izatnagar Dairy farm having an average population of about 1000 livestock was analyzed for sex-specific calf birth, abortions, still births and mortality were assessed with reference to the FMD outbreak years and non-FMD outbreak years. In the study period, two FMD outbreaks were recorded one in 2010 June (due to FMD virus type"O") and other in January 2016 (due to FMD virus type "A"). Results: In years of FMD outbreak (2010-11 and year 2015-16), there were no biases of the disease towards sex and breed of the affected animals. However, FMD lesions were more apparent in calves and young stocks of Vrindavani strain. In years of FMD outbreaks on the farm male calf births were significantly more than female calf births (in 2010-11, male: female:: 1... more
by Bhoj Singh, Raj Karan, Vidya Singh
in Noto-are Medicine, March 14, 2014. (15185525)
Axone, a fermented soybean food of Nagaland, is a common side dish in North-East India and neighbouring countries. In the study 191 samples of axone collected from 7 tribes (44 Angami, 12 Ao, 36 Chakesang, 3 kuki, 62 Lotha, 2 Pochury and 32 Sema) were analyzed. The pH of matured axone (5.7 to 8.62) was significantly (p<0.001) higher than fresh (4.56 to 7.54) and dried axone (5.58 to 8.54). The pH of Sema axone (8.02) was significantly (p, 0.001) higher than average pH of axone of other tribes. Total aerobic mesophilic plate count (TAMPC) of axone ranged between 6.44 Log10 cfu/g and 12.98 Log10 cfu/g. The aerobic endospore count (AEC) in axone (5.6Log10 to 10.55 Log10 cfu/ g) had strong correlation with TAMPC (r, 0.41) and was the major constituent of it. Of the twelve species of Bacillus identified in axone five most common were B. coagulans (55.5%), B. pantothenticus (12.4%), B. subtilis (11.5%), B. stearothermophilus (8.4%) and B. lentus (6.3%). About one third (31.4%) axone samples were positive for coliforms... more
by GNANAJEYARAMAN RAJARAM, Dr.GUNASEELAN D, P.K. KUMARESAN
in Noto-are Computer engineering. Computer hardware, July 20, 2014. (19205225)
Mobile cloud computing platforms represent a more secure way for provisioning applications and online services to users over mobile networks. Mobile cloud provisioning takes advantage of the inherent benefits of cloud computing for monitoring, security detection and malware prevention capabilities to protect its mobile users. Security and privacy preservation in is the main expectation of the cloud users in Mobile based cloud servicing. In this project, cloud based secure mobile health care model with feedback decision support is proposed. This mainly aims to improve the quality of the health care with reduced complexity and no compromise in security. This paper also mentions the difficulty imposed on client privacy and monitoring the service provider. To protect the client privacy, Boneh-Franklin Identity based encryption is proposed to reduce the decryption complexity due to the use of IBE (Identity based encryption) technique. To protect mobile Health (m-Health) service providers programs, the branching tree is expanded by random permutation and the decision thresholds used at the decision branching nodes are also randomized... more
by MEENALOCHINI J, Dr.C.Florence Annal Annal
in Noto-are Geography. Anthropology. Recreation, November 8, 2017. (15135108)
The main aim of the study is to evaluate ground water quality suitable for irrigation purpose in Dindigul Corporation, Tamil Nadu, India. In the study area 30 water samples were collected to determine the physical parameters and chemical parameters. Sodium Adsorption Ratio(SAR), Sodium percentage(SP), Potential Salinity(PS), Residual Sodium Carbonate(RSC), Kellys Ratio and Soluble Sodium Percent (SSP)have been determined for analyse the irrigation water quality. From the analysis result reveals that SAR value indicate 84% of excellent13% of good and 3% of unsuitable water category. Based on sodium percent 10% are good, 50% are permissible and 40% are unsuitable for irrigation. Based on PS and RSC ratio about 97% comes under unsuitable water and 3% are suitable water for irrigation. Kellys Ratio and SSP valuesindicate 27% of good quality and 73% are unsuitable irrigation water. The high concentration of salinity found in western part of the study area. The result shows that most of the samples are unfit for irrigation.
by Bhoj Singh
in Noto-are Medicine, June 2, 2014. (12446517)
Integrated farming is popular in India but little is understood about circulation of multi-host pathogens under the emerging farming systems (IFS). Circulation of Edwardsiella a pathogen of public health importance has been studied in IFS. Gill swabs of fish (8), pond water (8), rectal swabs of pig (36) and cloacal swabs of duck (32) from an IFS and 24 samples each of rectal swabs of pigs and cloacal swabs of ducks from the down colony (300 m on down side from the farm) were analyzed for the presence of edwardsiellae and heterogeneity among the isolates. A total of 24 E. tarda and 6 E. hoshinae were isolated. No E. hoshinae was isolated from the IFS. We isolated both E. tarda and E. hoshinae from ducks but no E. tarda in down colony pigs. Though 55% E. tarda from the IFS were multi-drug-resistant (MDR) type, no MDR strain was detected in the down colony. Plasmid(s) were detected in E. tarda but not in E. hoshinae isolates. The study concluded that E. tarda may be detected in all components of the IFS but E. tarda from different sources may not be of similar type... more
by Bhoj Singh
in Noto-are Medicine, September 19, 2017. (12424858)
Over last six years (April 2011 to August 2017), of the 2968 bacterial isolates from different clinically sick animals and their environment tested for their antimicrobial sensitivity (by disc diffusion assay and E-test as per CLSI), 480 were resistant to carbapenem drugs (meropenem or imipenem or ertapenem). On further characterization of the 480 carbapenem-resistant bacteria, 124 were phenotypically characterized as Metallo-B-lactamase (MBL) producers using double disc diffusion assay and E-Test (for imipenem and imipenem+EDTA) and 51 were genotypically confirmed carrying one or more known Carbapenemase genes (Figure 1). Of the 51 genotypically MBL positive 43 were confirmed to carry New Delhi Metallo-B-lactamase (NDM), one Acinetobacter lowffii carried Klebsiella pneumoniae carbapenmase (KPC), two Shewanella sp. strains were positive for Verona integrom mediate carbapenemase (VIM), four strains (2 of Escherichia coli, one each of Aeromonas bestiarum and Raoultella terrigena) carried OXA beta-lactamases (OXA) and one E... more
by Bhoj Singh
in Noto-are Medicine, September 21, 2017. (15168183)
Pasteurella infections in horses are not frequent and only a few Pasteurella have been reported causing infection in horses. Pasteurella rarely causes any fatal infection in horses but in association with other bacteria or virus may be problematic. Since March 2011 to September 2017 we encountered only two cases where Pasteurella strains could be detected. In one case horse with conjunctivitis and ocular discharges had mixed infection of Pasteurella dagmatis and Streptococcus equi ssp. zooepidemicus, and in another case of strangles, Pasteurella caballi could be isolated along with Streptococcus equi ssp. equi. In both, the cases Pasteurella isolates were sensitive to most of the commonly used antibiotics. Both the cases were cured after strepto-penicillin treatment.
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